Isolation of bovine coronavirus (BCov) in cell cultures

One of the most common viruses in world that causes disease cattle is bovine coronavirus (BCoV). This virus causative agent respiratory and gastrointestinal infections newborn calves, resulting significant economic losses both dairy meat farming. Considering complex epizootic situation with coronaviruses partial antigenic affinity BCoV other species animals humans, isolation new strains coronaviruses, their identification optimization cultivation conditions becomes extremely important relevant. The aim our research was to determine features methods select for its cell culture order obtain highest titers infectious activity. Isolation performed monolayers MDBK primary-trypsinized calf kidney cells, using 20 samples collected from calves clinical signs or/and disease. 16 were positive by means Real-Time PCR test. Up fifth serial passage, only 4 these isolates presented typical syncytial cytopathic effect. It has been experimentally established continious line (MDBK) (CK) are suitable accumulation. titer at level passage cultures CK cells reached 5.54 ± 0.16 lg TCD50/ml 5.59 0.14 TCD50/ml, respectively. However, due high cost obtaining cultures, this method may be unacceptable pharmaceutical companies laboratories. Also after 5 passages, viral material again examined confirm - study a characteristic CPE had result PCR. PCR-positive 12 samples, could not isolated continuous Vero, as well lung cultures. fact indicate presence different circulation farms country. Further planned focused on optimizing modes isolation, identify cultural properties circulating Ukraine. We will also continue obtained isolate subsequent development tools diagnosis immunoprophylaxis infection veterinary medicine.